the term tune up refers to an engine not running as it should. even a dirty air filter will affect fuel consumption. low inflated tyres. roof racks add drag using fuel as would an open window.
even sensor such as MAF deteriorate over time and get dirty.
the gas burnt by being run out of tune varies considerably depending on state of tune.
Alot is not a word! A lot is two words, not one!
Lose 3 miles, not loose! Can't you losers use spellcheck?
probably you will loose 3 miles per gallon